https://ednaresources.science/lessonssource daily 0.75 2018-08-08 https://images.squarespace-cdn.com/content/v1/5b0346f596d455233a755d2c/1530136281618-JIFPVJL75XBB2YUA6W65/filter_tubes.JPG lessonsSource https://ednaresources.science/lessonssource/2018/6/18/field-sample-collection-tips-and-tricks monthly 0.5 2019-05-16 https://ednaresources.science/lessonssource/2018/6/18/sampling-design-tips-and-trick monthly 0.5 2019-05-16 https://ednaresources.science/lessonssource/2018/8/8/communicating-about-uncertainty-km2ry monthly 0.5 2019-05-16 https://ednaresources.science/field-methods daily 0.75 2022-05-20 https://images.squarespace-cdn.com/content/v1/5b0346f596d455233a755d2c/95d042d1-541a-4d25-81f3-ecba427591a9/MiFish+manual.jpg Field Methods - environmental dna sampling and experiment manual The eDNA Society Minamoto et al. 2020 https://images.squarespace-cdn.com/content/v1/5b0346f596d455233a755d2c/1551280807240-3YI14NI9H1OLW89XLPIJ/Leeselab.jpg Field Methods - eDNA sampling in the rivers Ruhr and Mӧhne LeeseLab https://images.squarespace-cdn.com/content/v1/5b0346f596d455233a755d2c/1549658346156-1UKGVZ03Y7VCT1T7J6MA/ADAS_survey.jpg Field Methods - Great Crested Newt eDNA Survey ADAS https://images.squarespace-cdn.com/content/v1/5b0346f596d455233a755d2c/1548179631509-0143P5UB06SOXC6NCIRQ/Test.jpg Field Methods - filtering water to capture DNA from aquatic organisms USGS 2015 https://images.squarespace-cdn.com/content/v1/5b0346f596d455233a755d2c/1551280764293-1XOJP3LW3O71FKE9BSMQ/USACE.jpg Field Methods - Filtering water samples for eDNA analysis USACE https://images.squarespace-cdn.com/content/v1/5b0346f596d455233a755d2c/1557961225514-034D4TTSJZTE2PUWEKXN/filtering.jpg Field Methods - eDNA Protocol for Sample Collection WSU 2017 https://images.squarespace-cdn.com/content/v1/5b0346f596d455233a755d2c/1549658536707-IWSQSV9OANRBJFX3VIBR/platypussSPOT.jpg Field Methods - eDNA sampling method platypussSPOT https://images.squarespace-cdn.com/content/v1/5b0346f596d455233a755d2c/1551280721589-KXCRYVGXG6ESZRXHX7Z4/RMRS%2Bcover.jpg Field Methods - A protocol for collecting environmental DNA samples from streams RMRS 2016 https://images.squarespace-cdn.com/content/v1/5b0346f596d455233a755d2c/1551280781396-CVTAFZQBGPAQXMA1G0KT/WSU.jpg Field Methods - Collecting environmental DNA from water samples WSU https://images.squarespace-cdn.com/content/v1/5b0346f596d455233a755d2c/53e10310-8a03-4cf5-9b42-86f4076c7639/QAPP.jpg Field Methods - quality assurance project plan for edna monitoring USFWS 2014 https://images.squarespace-cdn.com/content/v1/5b0346f596d455233a755d2c/1549657252057-Q9T5J2I2G8NKUVMZAOGF/Genidaqs_cover.jpg Field Methods - Field collection procedure for aquatic eDNA sampling and analysis GENIDAQS 2017 https://images.squarespace-cdn.com/content/v1/5b0346f596d455233a755d2c/1551280746754-HR8UVL8Y493U9WQ2EQWW/GCN_sampling.jpg Field Methods - Analytical and methodological development for improved surveillance Freshwater Habitats Trust 2014 https://images.squarespace-cdn.com/content/v1/5b0346f596d455233a755d2c/1530219673197-6SE91RRRSWD5OCLVNXRG/eDNA+pic1.jpg Field Methods https://ednaresources.science/edna-labs daily 0.75 2023-04-17 https://images.squarespace-cdn.com/content/v1/5b0346f596d455233a755d2c/1530221782288-YWZVIT44X6KT9CT6HE0N/IMG_2376.JPG eDNA Labs https://ednaresources.science/web-tools daily 0.75 2022-03-22 https://images.squarespace-cdn.com/content/v1/5b0346f596d455233a755d2c/1530222094936-K9I27Z7RN7JVEKH9QZOX/IMG_1311.JPG Web Tools https://ednaresources.science/intro daily 0.75 2022-03-23 https://images.squarespace-cdn.com/content/v1/5b0346f596d455233a755d2c/4029febc-48be-41b6-aa07-e6b82ff48b99/Peterson+pond+sampling+site+-+AZ.jpg Intro to eDNA - Make it stand out Whatever it is, the way you tell your story online can make all the difference. https://images.squarespace-cdn.com/content/v1/5b0346f596d455233a755d2c/72c9f262-5f3c-4588-ba04-5e996fa6e0b5/Yaquina_Head_tide_pools%2C_sculpin_%2827880997525%29.jpg Intro to eDNA - Target species detection This website focuses on species-specific eDNA detection, which is highly reliable for answering management-relevant questions, particularly for rare species. However, many of the materials here are relevant to eDNA metabarcoding approaches as well. https://images.squarespace-cdn.com/content/v1/5b0346f596d455233a755d2c/1530221054743-VXQPQQJD79FQ1KKF3D0L/stebbinsi_measure.JPG Intro to eDNA https://ednaresources.science/bullfrog-eradication daily 0.75 2022-05-20 https://images.squarespace-cdn.com/content/v1/5b0346f596d455233a755d2c/1527711774069-4SNRUSLLXN8ZMBON4HTK/Aquatic_environment_swamp_water_landscape-1098x461.jpg Bullfrog Eradication https://images.squarespace-cdn.com/content/v1/5b0346f596d455233a755d2c/1530001184788-DH1BCKXEQL54212O0EXZ/Bullfrog_wikimedia_needs_attribution.jpg Bullfrog Eradication https://ednaresources.science/reference-lists daily 0.75 2022-03-24 https://images.squarespace-cdn.com/content/v1/5b0346f596d455233a755d2c/6798cd6e-54b7-4cc5-9c00-e9ac75cde666/library2.jpg Reference Library https://images.squarespace-cdn.com/content/v1/5b0346f596d455233a755d2c/1530219212510-S0TE1XF2PYSIM282QMEV/20160923+-43.jpg Reference Library https://ednaresources.science/sonora-tiger-salamander daily 0.75 2022-05-20 https://images.squarespace-cdn.com/content/v1/5b0346f596d455233a755d2c/1530131373434-YURB847S6ZJ8F20O6KPX/Sonora+tiger+salamander+eggs.JPG Sonoran Tiger Salamander Monitoring https://images.squarespace-cdn.com/content/v1/5b0346f596d455233a755d2c/1530130225710-PH5T3LF6NZY9O5XP710B/IMG_4856.jpg Sonoran Tiger Salamander Monitoring https://images.squarespace-cdn.com/content/v1/5b0346f596d455233a755d2c/1530131816883-CW0TMAZ315C1RWHZDRPO/stebbinsi_seining_cows.JPG Sonoran Tiger Salamander Monitoring https://ednaresources.science/edna-resources daily 1.0 2019-05-08 https://images.squarespace-cdn.com/content/v1/5b0346f596d455233a755d2c/1529906410893-X36KG34S7JRRVU3HD44Q/Under+Water002.jpg eDNA Resources https://images.squarespace-cdn.com/content/v1/5b0346f596d455233a755d2c/1530302093051-ISTYMJEMG1HHKC9G9457/Collecting+eDNA+water+samples+-+WA.jpg eDNA Resources https://images.squarespace-cdn.com/content/v1/5b0346f596d455233a755d2c/1529906508644-DY94ELLI7IEGNTU9L8HG/Lab003.jpg eDNA Resources https://images.squarespace-cdn.com/content/v1/5b0346f596d455233a755d2c/1557353867180-1054LYFF664SNYLUUJUT/Meadow%2Bcrop.jpg eDNA Resources https://ednaresources.science/project-profiles daily 0.75 2022-03-23 https://images.squarespace-cdn.com/content/v1/5b0346f596d455233a755d2c/1527711234148-A2BILXRICTI8W9395YVH/Tiger-Salamander-800x483.gif Project Profiles https://images.squarespace-cdn.com/content/v1/5b0346f596d455233a755d2c/1527711320911-R4MCYGYXLR7XI0Q9UCJV/AZ-tree-frog.gif Project Profiles https://images.squarespace-cdn.com/content/v1/5b0346f596d455233a755d2c/1527711139946-5H1BYD086402UGEPZ32R/NorthAm-bullfrog.gif Project Profiles https://images.squarespace-cdn.com/content/v1/5b0346f596d455233a755d2c/1530051117530-G1ZM5CXIBYYFI6BKGRQ7/From+Cori.jpg Project Profiles Picture credit: Michel Ohmer https://images.squarespace-cdn.com/content/v1/5b0346f596d455233a755d2c/1530221279131-ZSDOYXZOIL1H8OVI035B/dipnetting_turkey_tank.JPG Project Profiles https://ednaresources.science/assay-list daily 0.75 2023-04-17 https://images.squarespace-cdn.com/content/v1/5b0346f596d455233a755d2c/1530221700684-C3I1NGZBW2ZZZS55NWLZ/wrightorum_rocks_Meryl.JPG Assay List https://ednaresources.science/faqs daily 0.75 2022-03-24 https://images.squarespace-cdn.com/content/v1/5b0346f596d455233a755d2c/946b6512-91e7-4d7c-a4e9-56a395a8c0db/eDNA+wordcloud.jpg FAQs - Frequently Asked Questions https://images.squarespace-cdn.com/content/v1/5b0346f596d455233a755d2c/1530221330715-7TCMTND9ZHY6FFXR2V2K/fish2001_NOAA_Danilo_Cedrone.jpg FAQs https://ednaresources.science/lab-analysis daily 0.75 2022-05-20 https://images.squarespace-cdn.com/content/v1/5b0346f596d455233a755d2c/1529906979423-NBN8AEA8PHXBOEMNGKAK/Capture.PNG LAB ANALYSIS Cornell eDNA Overview https://images.squarespace-cdn.com/content/v1/5b0346f596d455233a755d2c/1529906952248-FW7XDTROH137LGRTOBWC/Capture.PNG LAB ANALYSIS WSU eDNA Lab Overview https://images.squarespace-cdn.com/content/v1/5b0346f596d455233a755d2c/1557355384970-LSC2PEUFJ0XS35JE797G/filter_tubes.JPG LAB ANALYSIS https://ednaresources.science/videos daily 0.75 2022-03-23 https://images.squarespace-cdn.com/content/v1/5b0346f596d455233a755d2c/dde00cb2-1a49-466d-8b70-015a459b17c2/MiFish+video.jpg Informational Videos - environmental DNa experiment manual UN Environment Programme Northwest Pacific Action Plan 2022 https://images.squarespace-cdn.com/content/v1/5b0346f596d455233a755d2c/8fe71419-e444-4c12-bb13-fb2c0598a30c/Screenshot+Leeselab.png Informational Videos - Environmental dna - toward next-generation biomonitoring Leese Lab https://images.squarespace-cdn.com/content/v1/5b0346f596d455233a755d2c/054c6939-34ec-43cc-bcf8-b27c1da531a5/Screenshot+detect+pretty+much+everything2.jpg Informational Videos - How we can detect pretty much everything TED-Ed https://images.squarespace-cdn.com/content/v1/5b0346f596d455233a755d2c/687d2c09-5896-431e-93c1-2ae31dae6a87/Screenshot+NOAA.jpg Informational Videos - environmental DNA NOAA Ocean Exploration https://images.squarespace-cdn.com/content/v1/5b0346f596d455233a755d2c/fb926de8-a142-4ff5-bde5-d89c4bd8e0ad/Screenshot+minion.jpg Informational Videos - Real time testing of environmental dna samples University of Canberra https://images.squarespace-cdn.com/content/v1/5b0346f596d455233a755d2c/c5b733b8-44fa-4994-a1ca-55d5e5f2113b/Screenshot+UMWSC.jpg Informational Videos - detecting invasive species in the field: portable edna screening tool USGS Upper Midwest Environmental Sciences Center https://images.squarespace-cdn.com/content/v1/5b0346f596d455233a755d2c/1558134865776-7GEYO8VPGS3S30T9OL8F/CALeDNA.jpg Informational Videos - overview of environmental dna barcoding CALeDNA https://images.squarespace-cdn.com/content/v1/5b0346f596d455233a755d2c/6514fdf0-ec3a-4816-a558-6fef95bc19c7/Screenshot+Janosik.jpg Informational Videos - an overview of environmental dna Dr. Alexis Janosik https://images.squarespace-cdn.com/content/v1/5b0346f596d455233a755d2c/64b80bfa-854b-474c-8255-71d29d597bbd/EnviroDNA+video+screenshot.png Informational Videos - What is environmental dna? EnviroDNA https://images.squarespace-cdn.com/content/v1/5b0346f596d455233a755d2c/1530221560889-FQKGOGRTE5IC7R6NF281/yose2.jpg Informational Videos https://ednaresources.science/guidance daily 0.75 2022-03-23 https://images.squarespace-cdn.com/content/v1/5b0346f596d455233a755d2c/1379109b-c548-4aa9-87e6-e91c78ae8684/slide22b.jpg Guidance Throughout the project, be vigilant about avoiding contamination and recording and reporting details of your methods and results. https://images.squarespace-cdn.com/content/v1/5b0346f596d455233a755d2c/3affb1c5-8994-46db-90f6-aef18b7fd3ce/Slide6.JPG Guidance … including whether it’s likely to improve detection or efficiency. https://images.squarespace-cdn.com/content/v1/5b0346f596d455233a755d2c/a87f37dd-67cc-42be-96c9-e47d93c1f857/DFO+image.jpg Guidance - guidance on the use of targeted eDNA analysis for management of aquatic species Fisheries and Oceans Canada 2021 https://images.squarespace-cdn.com/content/v1/5b0346f596d455233a755d2c/557310b0-f487-4a43-b2ce-16aa96049e66/Slide9.JPG Guidance If your objective is biodiversity monitoring or you have a large number of target species, eDNA metabarcoding might be the best approach. https://images.squarespace-cdn.com/content/v1/5b0346f596d455233a755d2c/859d8baf-69e2-44ac-9c4c-1ac5fb8c03da/Slide11.JPG Guidance If your detection probabilities are influenced by any measured factors, you can adapt your sampling strategy to optimize detection and efficiency. https://images.squarespace-cdn.com/content/v1/5b0346f596d455233a755d2c/975fe4a9-95d4-4dec-8db1-8602a8b6de83/Slide5.JPG Guidance Assess whether eDNA sampling can give you the information you’re looking for. https://images.squarespace-cdn.com/content/v1/5b0346f596d455233a755d2c/1557960683791-FSWQTJJOJATO2KGXQZ68/IMG_1312.JPG Guidance - CRITICAL CONSIDERATIONS FOR THE APPLICATION OF eNVIRONMENTAL dna METHODS Goldberg et al. 2016 https://images.squarespace-cdn.com/content/v1/5b0346f596d455233a755d2c/1a2a9067-0ce0-42e0-83da-69315f4d383b/Slide18.JPG Guidance Environmental DNA sampling is unlikely to completely replace field surveys. Instead, eDNA sampling can be integrated into field surveys for target species. https://images.squarespace-cdn.com/content/v1/5b0346f596d455233a755d2c/f8c915a9-0c00-410e-bf5d-a353806ce110/Slide19.JPG Guidance Integrating eDNA and field surveys can address uncertainty in eDNA sampling results. https://images.squarespace-cdn.com/content/v1/5b0346f596d455233a755d2c/babf9c58-79dd-4705-b6bd-426853ee54bd/Slide3.JPG Guidance - A framework for planning eDNA sampling https://images.squarespace-cdn.com/content/v1/5b0346f596d455233a755d2c/425fc539-a251-48eb-92d2-212498a22a92/slide4+border.jpg Guidance Start with your overall objectives. Are you interested in detecting species presence, or do you need more information about individuals or populations? https://images.squarespace-cdn.com/content/v1/5b0346f596d455233a755d2c/5b6d5f71-0b03-4012-b2ab-0e65fe252f8e/Slide8.JPG Guidance Answering these questions can help you decide if eDNA sampling is the best tool for your project. https://images.squarespace-cdn.com/content/v1/5b0346f596d455233a755d2c/1530221945501-WKE6QC7IM9KPY7O7J4K9/eDNA+pic2.jpg Guidance https://ednaresources.science/lessons-learned daily 0.75 2018-06-29 https://images.squarespace-cdn.com/content/v1/5b0346f596d455233a755d2c/1557968293105-Y0WVUUZUA7BW2HH1M48A/bull_trout_sampling.jpg Lessons Learned https://ednaresources.science/developments daily 0.75 2022-03-22 https://images.squarespace-cdn.com/content/v1/5b0346f596d455233a755d2c/1530222935042-OHPY1EJYUIVGHSOTINM1/DSC_0032.JPG New Developments https://ednaresources.science/about-us daily 0.75 2019-07-02 https://ednaresources.science/style daily 0.75 2019-01-14 https://images.squarespace-cdn.com/content/v1/5b0346f596d455233a755d2c/1547478523137-DBBQATANROKDQ21COKML/test1.JPG Style - All about border collies https://images.squarespace-cdn.com/content/v1/5b0346f596d455233a755d2c/1547478783892-F1FLYR0ARL0KIN8L907D/test2.JPG Style - Herding in action https://ednaresources.science/reference-lists-1 daily 0.75 2022-03-23 https://images.squarespace-cdn.com/content/v1/5b0346f596d455233a755d2c/1530219212510-S0TE1XF2PYSIM282QMEV/20160923+-43.jpg Reference Lists (Copy)